The BioTeZ Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product.
The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22,4 Å to increase the distance between the target molecule and the biotin groups.