BIOTEZ

The BioTeZ Berlin-Buch GmbH is a Biotechnology company founded in 1992.
BioTeZ is partner of research institutions, the pharmaceutical industry and laboratory diagnostics.
Our sister company Steffens-Biotec, Ebringen (ISO 13485) is specialised in manufacturing CE in-vitro diagnostics.

BioTeZ Professional Assay Development & Manufacturing Tools

Immbolisation & Detection

Polystreptavidin R is a chemically modified polymerized Streptavidin characterized by an extraordinary high Biotin binding capacity.

Immbolisation & Detection
TA-Pas Labelling System Polystreptavidin Coating Solution Polystreptavidin Coating Kit Glass Streptavidin Coating Kit Streptavidin Poly-HRP Streptavidin Coated Microplates Biotin Labelling Kit

TA-Pas Labelling System

The TA-PAS System is a fast two-component protein-ligand interaction system. One component is the small synthetic label called “TA-Label” (<1000 Da). The second component is the “PAS-Antibody” which binds the “TA-Label” with high specificity and efficiency.
The TA-PAS System supports a large number of applications including immobilization, enrichment or detection of antibodies, other proteins, primers and other nucleic acids. The TA-PAS System is a simple yet elegant system to link TA labelled molecules in assays by exploiting the high affinity to PAS capture antibody. 

Polystreptavidin Coating Solution

Polystreptavidin R is a chemically modified polymerized Streptavidin characterized by an extraordinary high Biotin binding capacity. Polystreptavidin R coatings on solid phases offer a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in a biotinylated form. Coatings made of Polystreptavidin R combine the excellent binding capacity with a high chemo and thermo stability and a long shelf life. It is suitable for coatings of membranes, beads, biochips, plastics etc.

  • Easy to conduct
  • Excellent Biotin binding capacity
  • High chemo and thermo stability
  • Long shelf life time
  • For coatings of membranes, beads, biochips, plastics etc.

Polystreptavidin Coating Kit Glass

Streptavidin binds very tightly to the small molecule Biotin. Streptavidin coating of solid phases offers a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylated form. As a further development BioTeZ offers a polymerized Streptavidin, the Polystreptavidin R, characterized by an extraordinary high Biotin binding capacity. Coatings made of Polystrept R combine the excellent binding capacity with a high chemo and thermo stability. It is very suitable for coating membranes, beads, biochips, plastics, glass etc. Polystreptavidin R is part of this set of reagents, which has been supplemented by a special solution for pre-coating. 

Streptavidin Coating Kit

Streptavidin binds very tightly to the small molecule Biotin. Streptavidin coating of solid phases offers a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylated form.
As a further product BioTeZ offers a polymerized Streptavidin, the Polystreptavidin R, characterized by an extraordinary high Biotin binding capacity (MC coating = maximum capacity). Coatings made of Polystrept R combine the excellent binding capacity with a high chemo and thermo stability. It is very suitable for coating membranes, beads, biochips, plastics etc.
 Streptavidin is part of this set of reagents, which has been supplemented by a special solution for pre-coating. The additional pre-coating serves to improve the outcome and should be used especially for very inert surfaces to ensure a high quality coating. The coating procedure starts with the pre-coating step.

Streptavidin Poly-HRP

The Poly-HRP labelled recombinant Streptavidin is produced by covalent conjugation of recombinant Streptavidin to highly activated Poly-HRP (Horseradish Peroxidase). Poly-HRP labels are characterized by high measurement signal amplification and low unspecific bonding. It is used to bind biotinylated compounds. The conjugated Poly-HRP provides enzyme activity for detection using an appropriate substrate system. It is provided in a conjugate stabilizer containing a preservative.
Streptavidin conjugate concentration: ca.  5 µg/ml
Molecular ratio Poly-HRP/target: 3-5
Applications:

  • ELISA: a recommended dilution is about 1:1000
  • Dot Blot
  • Western Blot
  • Immunohistochemistry

Streptavidin Coated Microplates

12 x 8-well strips and frame, C well, polystyrene strips, clear, breakable (C8 Lockwell Maxisorp).This approach is especially useful for molecules which cannot be immobilised via passive adsorption (e.g. peptides, carbohydrates, haptens, hydrophilic compounds) or are denatured by random hydrophobic interaction. Applications include EIA techniques, DNA hybridisation assays, quantification of PCR products and receptor-ligand studies.

Biotin Labelling Kit

The BioTeZ Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product.
The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22,4 Å to increase the distance between the target molecule and the biotin groups. 

Products and Services

Affinity Chromatography

We support mykotoxin and vitaminanalysis using very effective immunoaffinity columns and ELISAs, and by developing toxin- and detection conjugates.

Primer/Oligonucleotide Synthesis

Long term experience in the field of DNA and RNA primer synthesis guarantees very high quality. We offer various services, fast shipment and ease your budget.

ELISA Technologies

Use our experience in the field of ELISA development, the production of tests and components. Test our innovation “recoveryELISA” for therapeutic drug monitoring.

Biotin-Streptavidin-Binding

Outstanding Biotin binding capacities using our high-tech molecule improve assay performance.

Human Proteases/
Proteins/Enzymes

Human proteases, ELISA & antibodies incl. the former Proteaimmun GmbH products and Services.

Biochemistry services

Extensive customer service in the field of immunochemistry like individual assay compounds or conjugates.

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Polystreptavidin R Surface Functionalization Kit

Polystrept R combines the excellent binding capacity with a high chemo and thermo stability.Additional pre-coating serves to improve the outcome and especially for very inert surfaces to ensure high quality coatings with Polystreptavidin R.

Polystreptavidin R provides product consistency and helps to reduce variability in diagnostic assays. Test it; make the comparison of BioTeZ Polystreptavidin R Coating Kit and surface activated beads or other surface activations. Typical applications where Polystreptavidin R is used: IVD assays, isolation and purification of biotinylated molecules, ligand coupling.

The BioTeZ Polystreptavidin R Coating Kit (Product Code: BTCK-MC) is useful for adsorptive coatings on microplates, beads, membranes etc. made of metal, nitrocellulose, ceramic, silicon, glass or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacrylate) PMMA or Polyamides with “Maximum Biotin Binding Capacity”. More Informations

New method for measuring effect of drugs

BioTeZ starts the project for the development of a companion diagnostic procedure. The aim is to determine the optimal drug dose for each patient to reduce side effects, to achieve the Therapeutic Window and to increase the effectiveness of the active ingredients by optimizing the dosage (Therapeutic Drug Monitoring). The project is being funded on behalf of the State of Berlin through the Program for the Promotion of Research, Innovations and Technologies (Pro FIT) and partially funded by the European Regional Development Fund (ERDF). More Informations